July , 2021, Volume : 2 Article : 9
DNA Barcoding: A Molecular Tool for Plant Biodiversity Assessment
Author : Rajesh Subramanian, Dhivya Karunamurthy, Poornima Ramalingam, Radhamani Thangavel, and Varanavasiappan Shanmugam
ABSTRACT
Plant biodiversity is an essential component of the ecosystem fabric. Presently, the biodiversity hotspots are being often subjected to habitat destruction, introduction of exotic species, overexploitation of species and profuse anthropogenic activity. The existing taxonomical way to identify, classify and Conserve plant species is often inadequate due to lack of taxonomic expertise. Hence, the alternative revolutionary approach based on biotechnological tool, DNA marker that was demonstrated success in animals using mitochondrial COI gene is deployed for plant species discrimination. However, plant DNA barcoding is a more complex, hence necessitates more than one barcode. Maturase K (matK) and ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) are recommended as standard barcodes with other supplementary barcodes in species discrimination in plants.
Keywords: DNA barcoding, matK, rbcL, genetic diversity
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