Cotton is one of the most important fiber and cash crop of India and plays a dominant role in the industrial and agricultural economy of the country. It provides the basic raw material (cotton fibre) to cotton textile industry. Cotton in India provides direct livelihood to 6 million farmers and about 40-50 million people are employed in cotton trade and its processing. In India, there are ten major cotton growing states which are divided into three zones, viz. north zone, central zone and south zone. North zone consists of Punjab, Haryana, and Rajasthan. Central zone includes Madhya Pradesh, Maharashtra and Gujarat. South zone comprises Andhra Pradesh, Telangana, Karnataka and Tamil Nadu. Besides these ten States, cotton cultivation has gained momentum in the Eastern State of Orissa. Cotton is also cultivated in small areas of non-traditional States such as Uttar Pradesh, West Bengal and Tripura. The cotton crop is grown extensively with a limiting factor, that is infected by fungal diseases like anthracnose (Collectotrichum gossipi), leaf blight (Alternaria macrospora), wilt (Fusarium oxysporum f.sp. vasinfectum), Ramularia leaf spot (Ramularia areola), root rot (Rhizoctonia spp.) etc. Out of all the diseases, root rot of cotton is the most devastating disease and now a days this disease has become a major limiting factor in cotton cultivation. The root rot caused by Rhizoctonia bataticola (Taub) Butler and Rhizoctonia solani (Kuhn) is one of the most serious diseases of cotton particularly in the northern region of India.