Jan , 2024, Volume : 5 Article : 1

Advancements in CRISPR-Based Genome Editing for Precision Plant Breeding

Author : Aditya Pratap Singh and Pramod Kumar Pandey


The field of CRISPR-based genome editing is undergoing continuous development, exerting a profound influence on precision plant breeding. This article delves into pivotal investigations and breakthroughs, encompassing a broad spectrum of applications ranging from bolstering disease resistance to enhancing crop yield. The discourse comprehensively addresses the seminal contributions of trailblazers such as Doudna and Charpentier, the expansion of base editing methodologies in rice and wheat, and the efficacy of the CRISPR/Cas9 system in facilitating high-efficiency multiplex genome editing across monocot and dicot plants. Furthermore, the article accentuates the imminent potential of CRISPR screens in plants, offering insights into strategic approaches and guidelines for optimal implementation. The thorough scrutiny of these advancements underscores the auspicious trajectory of CRISPR-based technologies in the transformation of plant biology and the enhancement of crop productivity.

Keywords: Base Editing; CRISPR/Cas9; CRISPR Screens; Crop Improvement; Genome Editing; Precision Plant Breeding.

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