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ABSTRACT

One of the technological solutions of the genomic age is the fluorescence-based, real-time, quantitative reverse transcription polymerase chain reaction (RT-PCR), which has become the preferred method for mRNA detection. A number of factors have aided the adaptation of this technology into a mainstream analysis method. The recent emphasis on nucleic acid quantification, combined with the advent of second-generation instrumentation and substitute chemistries, has aided the adoption of this technology in independent research laboratories. Consequently, it is widely used in functional genomics, forensics, microbiology, molecular medicine, virology, and biotechnological research. During global epidemic scenarios, the ability to rapidly confirm or clear suspected cases is critical, particularly when clinical symptoms are difficult to discern from other respiratory infections including influenza. Rapid and reliable diagnostic testing is critical in the wake of the pandemic COVID-19. Molecular diagnostics is critical for detecting the spreading novel corona virus. Early on in the outbreak, a number of suitable assays were made available, but the most sophisticated tool for detection now-a-days is the RT-qPCR (Real-Time Quantitative PCR).

Keywords: RT-PCR, SARS-CoV-2, Testing, Detection

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Real-time RT-PCR in SARS-CoV-2 detection issues affecting the results_compressed.pdf