The growing global population and persistent challenges of malnutrition necessitate innovative approaches to enhance the nutritional quality of major food crops. Genome editing technologies, particularly CRISPR-Cas systems, have emerged as powerful tools in the development of crops with improved nutritional profiles. These technologies enable precise, targeted modifications at specific genomic loci, allowing for the enhancement of desirable traits such as increased vitamin content, improved protein quality, and reduced levels of anti-nutritional factors. Unlike traditional breeding methods, which are often time-consuming and resource-intensive, genome editing provides a more efficient and flexible approach to crop improvement. This article highlights recent advancements in genome editing applications for bio-fortification in staple crops such as rice, wheat, and maize. Additionally, it explores the potential of these technologies to address global food security and malnutrition by developing crops that are not only higher yielding but also nutritionally superior. The integration of genome editing into modern breeding programs represents a promising strategy for meeting the nutritional demands of a rapidly growing population while also enhancing the resilience and sustainability of agricultural systems.